![]() Cap-independent translational control of carcinogenesis. The zipper model of translational control: a small upstream ORF is the switch that controls structural remodeling of an mRNA leader. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Translational regulation of gene expression during conditions of cell stress. Cleavage of polypeptide chain initiation factor eIF4GI during apoptosis in lymphoma cells: characterisation of an internal fragment generated by caspase-3-mediated cleavage. doi: 10.1002/wrna.1202īushell M, Poncet D, Marissen WE, et al. Wiley Interdiscip Rev RNA, 2014 5, 131−9. IRES mediated translational regulation of p53 isoforms. Identification of eukaryotic mRNAs that are translated at reduced cap binding complex eIF4F concentrations using a cDNA microarray. Identification of a motif that mediates polypyrimidine tract-binding protein-dependent internal ribosome entry. Mitchell SA, Spriggs KA, Bushell M, et al. Identification of cryptic putative IRESs within the ORF encoding the nonstructural proteins of the human rhinovirus 16 genome. Internal ribosome entry in the coding region of murine hepatitis virus mRNA 5. The human immunodeficiency virus type 1 gag gene encodes an internal ribosome entry site. Evidence that an IRES within the Notch2 coding region can direct expression of a nuclear form of the protein. Identification and characterization of a novel cell cycle-regulated internal ribosome entry site. Reversible induction of translational isoforms of p53 in glucose deprivation. The emerging picture of CDK11: genetic, functional and medicinal aspects. Adv Exp Med Biol, 2018 1049, 147−73.ĭos Santos Paparidis NF, Canduri F. ![]() Spinocerebellar ataxia type 6: molecular mechanisms and calcium channel genetics. Identification of internal ribosomal entry site inside open reading frame of 14-3-3β gene. Preferential translation of internal ribosome entry site-containing mRNAs during the mitotic cycle in mammalian cells. NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response. Identification of the internal ribosome entry sites (IRES) of prion protein gene. A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. Jang SK, Kräusslich HG, Nicklin MJ, et al. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Two internal ribosome entry sites mediate the translation of p53 isoforms. Internal ribosome entry sites in eukaryotic mRNA molecules. Conclusion Five IRESs are present in the CVB3 coding region. The cryptic promoter was also excluded by RT-qPCR. Two IRESs in VP2 (1461–1646 nt) and VP1 (2784–2983 nt) of P1 one IRES in 2C (4119–4564 nt) of P2 and two IRESs in 3C (5634–5834 nt) and 3D (6870–7087 nt) of P3 were identified according to Fluc/Rluc activity ratio. Results After transfection of full length or truncated sequences of the P1, P2, or P3 plasmids, six GFP-fused protein bands in P1, six bands in P2 and nine bands in P3 were detected through western blotting. Expression of Fluc and Rluc mRNA of the bicistronic vector was detected by RT-qPCR. The sequences of possible IRESs were inserted into specific Fluc/Rluc bicistronic vectors, in which the potential IRESs were determined according to the Fluc/Rluc activity ratio. After transfection, possible IRES-dependent green fluorescent protein (GFP)-fused proteins were detected by anti-GFP western blotting. ![]() Methods The sequences of P1, P2, or P3 of the CVB3 genome or the truncated sequences from each antithymocyte globulin (ATG) to the end of the P1, P2, or P3 gene were inserted into the pEGFP-N1 vector. Objective This study aimed to identify internal ribosome entry sites (IRESs) in the open reading frame (ORF) of the Coxsackievirus B3 (CVB3) genome. ![]()
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